Description

SPECIFICATION	DETAILS
Product Name	Kisspeptin-10 (KP-10)
Synonyms	Metastin (45-54), KISS1 (112-121), KP-10 peptide, KISS1R agonist
CAS Number	374675-21-5
PubChem CID	25240297
Molecular Formula	C₆₃H₈₃N₁₇O₁₄
Molecular Weight	1302.45 g/mol
Sequence (1-letter)	YNWNSFGLRF-NH₂
Sequence (3-letter)	Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂
Peptide Class	Decapeptide (C-terminally amidated)
Source	Synthetic (Solid-Phase Peptide Synthesis)
Purity	≥98% (HPLC verified)
Appearance	White to off-white lyophilized powder
Net Content	10 mg per vial
Format	Single sealed glass vial
Third-Party Tested	Yes — Janoshik Analytical
Storage (Lyophilized)	-20 °C, sealed, protected from light and moisture
Shelf Life	Up to 24 months under recommended storage
Catalog Number	KP10-10
Research Category	Neuroendocrine / Reproductive Research

Overview

Kisspeptin-10 is a synthetic decapeptide corresponding to amino acids 45–54 of metastin, the endogenous product of the KISS1 gene. This ten-residue sequence represents the minimum fragment required for full activation of the kisspeptin receptor, KISS1R, also referenced in the research literature as GPR54. Kisspeptin-10 has been used extensively as a reference agonist in in vitro and preclinical studies examining the hypothalamic-pituitary-gonadal (HPG) axis, gonadotropin-releasing hormone (GnRH) neuronal signaling, and broader reproductive neuroendocrinology.

Chemical Identity

The compound has the molecular formula C₆₃H₈₃N₁₇O₁₄ with a calculated molecular weight of 1302.45 g/mol and is registered under CAS number 374675-21-5. The amino acid sequence Tyr-Asn-Trp-Asn-Ser-Phe-Gly-Leu-Arg-Phe-NH₂ terminates in a C-terminal amide group, a structural feature reported in the literature as critical for receptor binding affinity. Kisspeptin-10 is produced by solid-phase peptide synthesis and supplied as a white to off-white lyophilized powder.

Research Applications

Published research has used Kisspeptin-10 as a probe compound in several laboratory contexts:

• Investigating KISS1R/GPR54 receptor signaling in transfected cell lines, including calcium mobilization assays and MAPK pathway activation studies.
• Characterizing pulsatile GnRH release from hypothalamic explants and GnRH-expressing neuronal cell models in preclinical research.
• Comparative potency assays against full-length kisspeptin variants (Kp-54, Kp-14, Kp-13) in receptor-binding and functional studies.
• In vitro tumor biology studies examining metastin/KISS1-mediated anti-migratory and anti-angiogenic endpoints in cancer cell line models.
• Reference standard in HPLC and mass spectrometry methodology for peptide characterization.

Quality & Testing

Each batch is supplied with a Certificate of Analysis (COA) generated by an independent third-party analytical laboratory (Janoshik Analytical). Analytical characterization includes reverse-phase HPLC for purity determination (≥98%) and mass spectrometry for identity confirmation. Batch-specific COA documentation is available on request.

Storage & Handling (Research Laboratory Reference)

Lyophilized Kisspeptin-10 powder should be stored sealed at -20 °C, protected from light and moisture. Under these conditions, reference literature indicates stability of up to 24 months from the date of manufacture. Reconstituted peptide solutions reported in published research were maintained at 2–8 °C for short-term laboratory use, and repeated freeze-thaw cycles were avoided in standard research workflows. Handle using appropriate aseptic technique in accordance with laboratory SOPs.

Research Background 

Discovery & Historical Context

The KISS1 gene was first identified in 1996 at the Penn State College of Medicine in Hershey, Pennsylvania, where it was initially characterized as a metastasis suppressor in melanoma cell lines. The gene was named in reference to its place of discovery (Hershey’s Kisses). The protein product, metastin, is a 145-amino-acid precursor that is proteolytically processed into several bioactive fragments of varying length, collectively referred to as the kisspeptins — Kp-54, Kp-14, Kp-13, and Kp-10.

The reproductive significance of the kisspeptin system was established in 2003, when independent research groups reported that loss-of-function mutations in KISS1R produced hypogonadotropic hypogonadism and failure of puberty in human cohorts and in knockout mouse models. This finding positioned the kisspeptin/KISS1R system as a central regulator of the hypothalamic-pituitary-gonadal axis and opened an active area of reproductive neuroendocrinology research.

Structural Characteristics

Kisspeptin-10 represents the C-terminal ten residues of metastin and retains full in vitro receptor-binding activity because the KISS1R recognition motif is contained within this fragment. The C-terminal amide group on the final phenylalanine residue is a defining feature; truncated variants lacking this amidation show substantially reduced receptor activation in published assays. Among the kisspeptin family, Kp-10 has been reported to exhibit binding affinity comparable to, or modestly exceeding, that of full-length metastin in GPR54-transfected cell systems.

Receptor Signaling (Research Findings)

KISS1R is a Gₑₐₐ — Gq-coupled — G-protein coupled receptor. In cell culture models, binding of Kisspeptin-10 to KISS1R has been reported to activate phospholipase C (PLC), hydrolyze PIP₂ into inositol trisphosphate (IP₃) and diacylglycerol (DAG), and mobilize intracellular calcium. Downstream activation of protein kinase C (PKC) and the mitogen-activated protein kinase (MAPK) cascade has also been documented. In hypothalamic neuronal models, this signaling has been associated with depolarization of GnRH-expressing neurons and modulation of GnRH release patterns in preclinical research.

Analytical Characterization

Research-grade Kisspeptin-10 is typically characterized using reverse-phase high-performance liquid chromatography (RP-HPLC) for purity, electrospray ionization mass spectrometry (ESI-MS) or MALDI-TOF for molecular identity, and amino acid analysis or Edman degradation where sequence confirmation is required. The Peptide Minds supply of Kisspeptin-10 is verified by RP-HPLC to ≥98% purity, with batch-specific certificates issued by an independent analytical laboratory.

Comparative Notes Within the Kisspeptin Family

Published comparative studies have examined Kp-10 alongside longer variants (Kp-54, Kp-14, Kp-13). All share the same C-terminal ten residues and activate KISS1R through the same molecular interface. Reported differences across variants relate primarily to in vitro stability, clearance in preclinical models, and distribution characteristics rather than intrinsic receptor potency. Kp-10 is frequently selected in research settings where a short, well-defined reference agonist is required for signaling assays.

Compliance Statement 

RESEARCH USE ONLY (RUO). This product is a research compound supplied for in vitro laboratory and preclinical research purposes only. It is not a drug, food, cosmetic, or dietary supplement, and has not been approved by the FDA or any other regulatory agency for diagnostic, therapeutic, or any human or veterinary use. It is not intended for human or veterinary consumption, administration, or clinical application of any kind. Purchase of this product requires the buyer to confirm they are a qualified researcher or institution and will use the product solely in a controlled laboratory setting in accordance with all applicable laws and regulations. Keep out of reach of children and non-research personnel.